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Contact Us

NBIP Ireland
Research Office
121 St Stephens Green
Dublin 2
Ireland

nbipadmin@rcsi.ie
+353 1 402 8651

Equipment access: please email contact person or research engineer/technician as outlined below:

Structured Light Illumination (Optogrid) Microscope with Volocity

Information:
Features include:
- Optigrid structure illumination
- Z stage piezo electric focus system
- Volocity acquisition and processing software
- Anti-vibration table
Objectives: 20x air, 40x air, 60x oil; Light source: epifluorescence cool LED white source; Filters: DAPI, FITC, TRITC filters sets for epifluorescence
Contact: Centre for Microscopy and Imaging email: This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Location: Centre for Microscopy and Imaging, NUIG

Fluoview 300 Laser Scanning Confocal Microscope

Information:
Features include:
- Inverted microscope fitted with an incubator for live cell experiments
- Dual monitor system used to view PMT collected fluorescent/DIC images
- Fluorescence and DIC Imaging
Objectives: 20x air, 40x air, 60x oil; Light sources: Gas laser system contains blue argon laser 488 nm, green helium laser 543 nm, red helium neon laser 633nm and epifluorescence white light source;  Filters: DAPI, FITC, TRITC filters sets for epifluorescence; Scanning Modes: 1 diemsional (spot scan) to 4 dimensional (x,y,z,t)
Contact: Centre for Microscopy and Imaging email:  This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Location: Centre for Microscopy and Imaging, NUIG

Andor Revolution Spinning Disk Confocal Microscope

Information:
Features include:
- Spinning disk confocal (Yokagawa CSU22)
- Fluorescence, DIC and Phase contrast imaging
- Incubator for live cell experiments
- Andor iq 2.3 acquisition software
- Piezo controlled stage
- High-resolution EMCCD camera (Andor iXonEM+)
Objectives: 10x, 20x, 40x air, 40x water, 60x water, 60x oil; Light source: 4 laser combiner, 4 solid state laser lines namely 405 nm, 488 nm, 561 nm, 640 nm and epifluorescence white light source; Filters: DAPI, FITC, TRITC filters sets for epifluorescence and range of emission filters with automatic shuttering; Incubator: varaible temperature/CO2 humidified incubation chamber; Stage:piezo controlled z-stage compatible with 35mm petri dishes, chamber slides and standard microscopy slides. Stage inserts are also available for multiwell plates.
Contact: Centre for Microscopy and Imaging email:  This e-mail address is being protected from spambots. You need JavaScript enabled to view it This e-mail address is being protected from spambots. You need JavaScript enabled to view it  
Location: Centre for Microscopy and Imaging, NUIG

Hitachi S2600N Variable Pressure Scanning Electron Microscope

Information:
Features include:
- Automatic beam axis-alignment functions (Auto Beam Setting, Auto Axial Alignment)
- High SE resolution of 10nm operating from 0.5-30kV.
- Backscatter BSE resolution 20nm at 25kV.
- Environmental chamber operating in variable pressure range from 1 to 270 Pa.
- Magnification of 15X to  200,000X Standard 40mmx40mm Specimen Stage.
- Dynamic Focus, Digital Beam control, Raster rotation, Measurement function.
- High probe currents for imaging at selected lower accelerating voltages.
- Real time, dual image display and signal mixing Mechanical stage with high tilt (-20 ~+70).
- Scanning speeds from 0.033 s to 20/24 s Associated 3D reconstruction Alicona MeX software. Contact: Centre for Microscopy and Imaging email This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Location: Centre for Microscopy and Imaging, NUIG


Hitachi H7000 Transmission Electron Microscope

Information:
Features include:
- Accelerating Voltage range from 25 to 125kV.
- Magnification range from 50X to 1000X in low mag. mode. 1000X to 500000X in zoom mode.
- Resolution of 10 Angstroms point to point.
- Heated biased hairpin tungsten filament Electron Gun.
- Eucentric side entry goniometric stage. 160mm phosphor viewing screen with 110mm focus screen incorporating image shift correction.
- 5 stage imaging lens system with image rotation.
- 3 stage condenser lens system with variable spot size from 0.3 to 8 microns.
- AMT digital image acquisition and image file archiving system
Contact: Centre for Microscopy and Imaging  email This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Location: Centre for Microscopy and Imaging, NUIG



Swept Source Optical Coherence Tomography System
Information:
This is a non invasive optical imaging modality that provides real time, 1D depth, 2D cross sectional and 3D volumetric images with micron level resolution and millimeters of imaging depth. Based on light scattered from different layers of material within the sample, OCT can provide structual information. OCT achieves higher resolution than ultrasound through the use of near infared wavelengths
Contact: Centre for Microscopy and Imaging  email This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Location: Centre for Microscopy and Imaging, NUIG


Tissue Viability Imager TiVi
Information:
The technology is based on polarization spectroscopy measurement of the absorption of light by the red blood cells within the green and red wavelength regions. By use of polarized light it is possible to 'see through' the top layer of the skin and probe the underlying dermal layer for information about the microvascular system.
-Quantification of RBC concentration
Contact: Centre for Microscopy and Imaging  email This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Location: Centre for Microscopy and Imaging, NUIG
 
Multi-modal photoacoustic system with co-registered ultrasound tomography
Information:
The photoaoustic technique is based on detection of lutrasound waves generated in tissue by the absorption of optical pulses (time-domain) or modulated light (frequency-domain) followed by the thermoelastic expansion of the absorbing volumes. The photoacoustic system uses absorption as contrast mechanism thus we can use as imaging (microcirculation) and spectroscopy (SO2 mapping). This is a cutting edge imaging modality for visualising samll animal anatomy and analysing function in vivo and non-invasively.
-High sensitivy and high specificity real time 3D imaging
-Quantification of hemoglobin content and oxygen saturation
-Microvascular hemodynamics in real time
-3D volumetric assessment

Contact: Centre for Microscopy and Imaging  email This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Location: Centre for Microscopy and Imaging, NUIG