Bio Apps


Biological Imaging

  • Biological Specimen Preparation for Imaging NBIPMOD22

    In order to achieve optimal conditions for imaging it is critical that the tissue samples that any investigator work with are of a high quality, that is, poorly prepared samples will result in the downstream images being of an equally poor standard regardless of the hardware used to acquire the images. This module will examine all levels of tissue preparation discussing important issues for each; 1) Transfection/infection of immortalized cells (HEK, MCFs, Helas..) for immunofluorescence and real time microscopy. 2) Preparation of primary neuronal (cerebellar, hippocampal, cortical) monolayers, the transfection or viral infection of these samples and the processing of these samples for immunofluorescence and real time microscopy. 3) The preparation of neuronal slice cultures (hippocampal, spinal) and the processing of these tissue samples to allow for 2-photon microscopy and immunohistochemistry. 4) The fixation and preparation of whole animals for immunohistochemistry. 5) And the processing of human samples for immunohistochemistry. Level 1; Course Director: Prof. David Henshall RCSI; Niamh Moran RCSI.

  • Tissue Optics and Microcirculation Imaging NBIPMOD25

    This is a basic tissue optics course which aims to provide the non-physical scientist with a good grounding in the physics of light interaction with human or animal tissue. Many of the same phenomena apply to other forms of radiation. The student will become familiar with scattering, absorption, anisotropy, albedo, de-polarisation etc. and their application to diagnosis and therapy. It also gives a short introduction to laser safety for those who might have occasion to design/operate laser based equipment. Level 1; Course Director: Martin Leahy UL. Further details on this course can be found here.

  • Small and Large animal imaging technologies NBIPMOD23

    This module comprises of two components. Firstly, an introduction to modalities of molecular imaging for biomedical applications is given which will contain: History & Perspectives; Pre Clinical Optical Imaging; Positron Emission Tomography (PET); Computed Tomography (CT); Single Photon Emission Computed Tomography (SPECT); Ultrasound Imaging; Magnetic Resonance Imaging; Multimodality Imaging: Case Study I-Oncology; Multimodality Imaging: Case Study II- CNS. Level 1; Course Provider: Dr.Annette Byrne, RCSI, Dublin. Phone: 01-4028673 email. The second part of this module will contain information on large animal imaging methodologies with specific relation to vascular system imaging. This content will be provided by Prof. Noel Caplice UCC. Due April 2011. email.

  • Multiparameter epifluorescence live cell imaging NBIPMOD18

    Fluorescence microscopy is a very powerful technique that allows for the imaging of single cell signalling processes using fluorescent probes tailored to be sensitive to certain changes of environmental conditions. This module is designed to teach investigators/students which have a fundamental understanding of the fluorescent probes and fluorescent microscopes how to apply this technology for live cell imaging. It will give insight in different types of fluorophores and their application in live cell imaging and how they can be used to provide detailed information on changes in ionic concentrations, membrane potentials, pH, reactive oxygen species at a single cell level. This module will also provide information on how 2 and even 3 fluorophores can be used in tandem allowing for the comparison of multiple events simultaneously. In addition the module will give background information to the widely used fluorescent proteins and their application of fluorescence (förster) resonance energy transfer in live cell imaging. Level 2;Course Directors: Markus Rehm RCSI; Valia VerriereRCSI.