confocal image

 

   Light Microscopy

  • Fluorescence Microscopy NBIPMOD20

    Fluorescence microscopy is a most widely used and versatile technique that allows for rapid data acquisition in both fixed and live samples. The sophisticated equipment in combination, sample preparation, dye loading, image processing and a lack of detailed education in the field can often lead to the acquisition of data that is inaccurate or misinterpreted. This module will provide technical understanding of the microscope, common imaging detectors, available fluorescent probes as well as digital imaging covering the following fields; Fluorescence excitation and emission, extinction and quantum yield; examples for sample preparation/staining; autofluorescence; filters, dichroics, and monochromators; multi fluorophor staining; Air and immersion objectives; resolution; CCD camera specifications; Digital imaging and image processing basics. At the end of the course students will be able to plan a simple experiment, generate images and controls, and interpret the imaging data. Level 1; Course Director: Tytus Bernas RCSI

    Module to be completed

  • Confocal Microscopy NBIPMOD21

    The goal of this course is to introduce the student to biological confocal microscopy. Part 1 includes a brief overview of the working principal of the confocal microscope and its component parts. This section also covers the key aspects of multidimensional image data generation including the principles of optical sectioning, different scanning techniques and spatial resolution. Part 2 is focused on the various applications of confocal microscopy as a powerful tool in the investigation of biological questions. This section encompasses techniques to study protein-protein interactions such as FRET and BRET as well as useful applications to study protein dynamics such as FRAP. Level 1; Course Director: Ruth Dooley RCSI

  • Advanced Confocal and 2 photon excitation microscopy NBIPMOD17

    Confocal and 2 photon excitation (2PE) microscopy are highly advanced techniques that enhance the resolution along the optical axis when compared to images acquired using epifluorescence microscopy. The advanced course will provide knowledge about the basic physics of the 2 photon excitation process identifying differences in the technology when compared to confocal microscopy. Here we will also emphasize the specific applications of 2PE microscopy whereby it allows for the monitoring cellular responses deep within tissues. Level: 2 Course DirectorHeiko Duessmann RCSI. This course is currently in preparation.

  • Introduction to Optics for Microscopy NBIPMOD2

    This module will focus on the geometrical and wave optics knowledge required to appreciate modern optical microscopy. After taking the module, participants will have a basic understanding of the following: refractive index, dispersion, Snell's law, refraction by a single optical surface, principal and marginal rays, raytracing, thin lenses, conjugate distance formulae, stops and pupils, optical aberrations, diffraction at an aperture, the point spread function, the Airy disc, depth of focus/field, magnifiers, objectives and eyepieces, the compound microscope, the confocal microscope.

    Indicative Learning Outcomes : On successful completion of this module, students will: Understand the principles and limitations of geometrical optics in imaging; Be able to trace rays through optical systems, graphically and numerically; Understand the effect of the primary aberrations on the point spread function; Understand Huygens-Fresnel principle for diffraction; Be able to calculate the diffraction-limited resolution of an imaging system; Know the basic layouts of the magnifier, compound microscope and confocal microscope.

    Level: 1 Course Director: Prof. Chris Dainty NUIG

  • Intermediate Optics for Microscopy NBIPMOD3

    This module focuses on the Fourier optics approach to the understanding of optical micropscopy. After completing the module, participants will have a knowledge of the following topics: the Fourier transform in twodimensions, properties of the Fourier transform, convolution, the sampling theorem, the Fourier theory of optical imaging, coherent, incoherent and partially coherent imaging, brightfield microscopy, tilted coherent and darkfield imaging, phase contrast methods, confocal microscopy, fluorescence techniques (outline only) and "super-resolution" methods. Level: 2A; ECTS: 2;Course Director: Prof. Chris Dainty NUIG.