Introduction to Optics for Microscopy

Introduction to Optics for Microscopy NBIPMOD2

This module will focus on the geometrical and wave optics knowledge required to appreciate modern optical microscopy. After taking the module, participants will have a basic understanding of the following: refractive index, dispersion, Snell's law, refraction by a single optical surface, principal and marginal rays, raytracing, thin lenses, conjugate distance formulae, stops and pupils, optical aberrations, diffraction at an aperture, the point spread function, the Airy disc, depth of focus/field, magnifiers, objectives and eyepieces, the compound microscope, the confocal microscope.

Indicative Learning Outcomes : On successful completion of this module, students will: Understand the principles and limitations of geometrical optics in imaging; Be able to trace rays through optical systems, graphically and numerically; Understand the effect of the primary aberrations on the point spread function; Understand Huygens-Fresnel principle for diffraction; Be able to calculate the diffraction-limited resolution of an imaging system; Know the basic layouts of the magnifier, compound microscope and confocal microscope.

Level: 1 Course Director: Prof. Chris Dainty NUIG