Introduction to Fluorescence Microscopy

Fluorescence Microscopy NBIPMOD20

Fluorescence microscopy is a most widely used and versatile technique that allows for rapid data acquisition in both fixed and live samples. The sophisticated equipment in combination, sample preparation, dye loading, image processing and a lack of detailed education in the field can often lead to the acquisition of data that is inaccurate or misinterpreted. This module will provide technical understanding of the microscope, common imaging detectors, available fluorescent probes as well as digital imaging covering the following fields; Fluorescence excitation and emission, extinction and quantum yield; examples for sample preparation/staining; autofluorescence; filters, dichroics, and monochromators; multi fluorophor staining; Air and immersion objectives; resolution; CCD camera specifications; Digital imaging and image processing basics. At the end of the course students will be able to plan a simple experiment, generate images and controls, and interpret the imaging data. Level 1; Course Director: Tytus Bernas RCSI

Module to be completed