Laser Scanning Confocal Microscopy

Confocal Microscopy NBIPMOD21

The goal of this course is to introduce the student to biological confocal microscopy. Part 1 includes a brief overview of the working principal of the confocal microscope and its component parts. This section also covers the key aspects of multidimensional image data generation including the principles of optical sectioning, different scanning techniques and spatial resolution. Part 2 is focused on the various applications of confocal microscopy as a powerful tool in the investigation of biological questions. This section encompasses techniques to study protein-protein interactions such as FRET and BRET as well as useful applications to study protein dynamics such as FRAP. Level 1; Course Director: Ruth Dooley RCSI