Synthetic Labelling of Polypeptides

Synthetic Labeling of Polypeptides NBIPMOD24

Peptides have an important potential in medicine, not only as investigation tools in life science research, but also as drug candidates in their own right and for the production of peptide conjugates and drug delivery systems. Current techniques of synthesis (Solid Phase) allow the convenient preparation of peptides comprising from 5 to about 40 amino-acids. These sequences can be assembled from natural, unnatural and/or post-translationally modified amino-acids and be modified with a variety of labelling agents (eg. fluorescent dyes, biotin).

This module will provide training in the techniques of:
- Solid Phase Peptide Synthesis (performed by an automated synthesiser)
- Peptide labelling (performed manually)
- Peptide isolation and purification by reverse-phase HPLC.
- Peptide lyophilization (Freeze-Drying)

Course director: Dr Marc Devocelle, Peptide Synthesis Laboratory, Department of Pharmaceutical & Medicinal Chemistry, Royal College of Surgeons in Ireland, 123 St. Stephen’s Green, Dublin 2, Ireland. T: +353 (0)1 402 2176

Further details on this course can be found here.